Phone : +964 (750) 733-5679

Birth Date: 1973-06-16

Nationality: Turkey

Address: Duhok- Avrocity

Mervan Yousif Huseyin

Lecturer

Department of Medical Microbiology


Speciality
Microbiology Medical Microbiology Antimicrobial activities.
Area Interest
Medical Microbiology Antibiotic resistance Antibacterial and Antifungal activities in addition searching for Antiviral and anticancer from Bacteria and natural sources
Teaching Materials
General Microbiology- Microbial Physiology - Air and soil Microbiology - Antibiotics - General Biology - Public Health

I received my PhD. in Microbiology from Damascus University in 2015 in Syria. I have a good experience in teaching and doing the research related to microbiology and Medical microbiology particularly in staphylococcus, bacillus subtilis, antibacterial extracts and antibiotic resistance. I am very interest in medical microbiology, biotechnology, water or food-borne pathogens such as meta-genomics, and any research concerning to medical microbiology and antibacterial subjects or searching for new antibiotics from Bacteria or natural resources. as well as, I am really interest to do search for new antibiotics and apply it against bacteria, water or food-borne pathogens, fungi, viruses and cancer cells. I have 8th researches published in international and local journals. As my area in Biology and Microbiology I have a good experience in Labs. in addition my working after graduation in the laboratories of pharmaceuticals, universities and schools. I have been in The University of Liverpool for two months in 2018 as an academic visit which supported and funded by both Applied Microbiology International (SFAM https://appliedmicrobiology.org) and Cara organization (the Council for At-Risk Academics) to get training in new methods in Microbiology. I have been in Newcastle University for two months in 2019 as an academic visit which supported and funded by Cara organization (the Council for At-Risk Academics-https://www.cara.ngo/ ) and get training in qPCR and Next Generation Sequencing NGS. I have dual Nationality (Syrian and Turkish) and I can teach in English.

Languages

1

Kurdish (Native)

2

Arabic (Proficient)

3

English (Proficient)

4

Turkish (Intermediate)

Skills

1

Skills

I received my PhD. in Microbiology from Damascus University in 2015. I have a good experience in teaching and good experience in doing the research related to medical microbiology, particularly in staphylococcus spp., bacillus subtilis, Enterobacteriaceae and antibacterial extracts. I have good knowledge in Enterobacteriaceae and antibiotic resistance, antimicrobial resistance. I am very interest in medical microbiology, biotechnology, water or food-borne pathogens such as meta-genomics, and any research concerning to medical microbiology and antibacterial subjects or searching for new antibiotics from Bacteria or natural resources. as well as, I am interest to do search for new antibiotics and apply it against bacteria, viruses and even cancer cell I really want to do research in water or food-borne pathogens, fungi, viruses. I have 8th researches published in international and local journals. As my area in Biology and Microbiology I have a good experience in Labs.

Education

2011 – 2015

Ph.D.

Microbiology

Damascus University

Medical Microbiology

2008 – 2010

M.S.

Microbiology

Damascus university

Medical Microbiology

2002 – 2003

B.Ed.

Education

Damascus University

Education, Methodology and Pedagogy

1995 – 1998

B.A.

Biology

Tishreen University

Biology and Biochemistry

Academic Title

2015-11-04

Lecturer

PhD. in Microbiology

Membership

I am supporting member in ASM

I am International member in this society, so the new Name is Applied Microbiology International and the old name was SFAM society for applied microbiology.

Publication Journal

2018-01-01

A broad-spectrum antibacterial activity of lyophilized crude extracts of Bacillus subtilis against clinical and food-borne pathogens

international Research Journal of Pharmacy and Medical Sciences : (Issue : 3) (Volume : 1)
2018

The aim of this research is to study the broad spectrum antibacterial activity of crude lyophilized extracts from Bacillus subtilis, then characterize these antibacterial activity. B. subtilis strains were isolated and identified by both API CHB 50 and molecular methods. Staphylococci strains, negative gram bacteria and Candida spp. were isolated from clinical and food samples and identified by biochemical and morphological tests. The crude antibacterial were extracted by flasks containing Lactose Broth LB, which incubated for 72 hours in shaker incubator at 37º ºC at 150 rev/min, then centrifuged 12000 rev/min or 15 min. at 4 ºC and filtrated the supernatants through 0.45 µm then frozen and lyophilized. The crude extracts were run on Diamonsil C18 column (5 µm, 250 × 4.6 mm) in HPLC system to separate the antibiotics and run on FTIR spectrum. Some strains of B. subtilis showed high activity against target strains, and others did not. The antibacterial activity of crude lyophilized extracts from B. subtilis (BS1A, BS2D, BS7SH, BS8SH ) against S. aureus strains was between high (16-40 mm) and intermediate (8-14 mm). While the antibacterial activity was intermediate (8-14 mm) against Gram negative and Candida spp. strains. Two kinds of antibiotics were separated from lyophilized extracts of B. subtilis and identified to the peptides families, Bacitracin A and Polymyxin B.

The antibacterial compounds, which are produced from Bacillus species, have a strong inhibition ability, and the composition of these compounds have not been elucidated. The aim of the present study is to explore the antibacterial activity of crude lyophilized extracts, then isolate and characterize these antibacterial. Bacillus subtilis strains were isolated from Damascus zone and identified by two methods which are the API CHB 50 and 16S DNA. Staphylococcus aureus strains were isolated from clinical samples, and identified by API Staph 20 and coagulase test. The crude antibacterial was extracted by flasks content LB where incubated for 72 h. in shaker incubator at 37°C at 150 rev/min, then centrifugated 12000 rev/minor 15 min. at 4 °C and filtrated the supernatants throw 0.45 µm, next frozen and lyophilized. The crude extracts were run on HPLC system to separate the antibiotics. Some strains of B. subtilis gave high activity against strains of S. aureus and others were not. Peptides families of antibiotics were separated from lyophilized extracts of B. subtilis, and then they were identified as bacitracin A and polymyxin B. This crude extract could function as antibacterial activity against S. aureus pathogens.

2016-01-01

Prevalence, Antibiotic resistance of Staphylococcus aureus, CNS and determination of MRSA, MRCNS strains in clinical samples

Tishreen University Journal -Biological Sciences Series : (Issue : 1) (Volume : 38)
2016

A total of 113 strains of S. aureus and CNS were isolated from clinical samples from three hospitals in Damascus city between 6/2013 – 8/2014, and were identified by biochemical and molecular tests. The percentage of S. aureus was (65.48%), followed S. epidermidis (25.66%) then S. saprophyticus (4.42%), so the prevalence of S. aureus was major in (pus, abscess) which was 28.37%, while the prevalence of S. epidermidis was more in CSF which was (31.03%) The molecular methods and antimicrobial resistance were studied in 55 strains of collection of samples (40 S. aureus and 15 CNS), where detected 16SrRNA, gap, and nuc genes for adjust the identification. Antibiotic resistance tests examined for 8 different antibiotics as follows: AMC (65%), GN (30%), CRO (62.5%), PE (7.5%), OFX (7.5%), AM (70%), VA (2.5%) and AX (55%). The percentage of presence of MRSA strains was 15% and MRCNS was 6.66%.

The current work aimed to study the distribution of B. subtilis and B. cereus in soils of Damascus zone and focused on the antibacterial activity of Bacillus species against Staphylococci species which were isolated from pathogen and soil’s samples. The study showed that the rate of the distribution of B. subtilis was (65.71%) and it was more than the rate of distribution of B. cereus which was (35.29%) in the soil, and the rate of distribution of Staphylococci were between (10 – 20%). The antibacterial activity was studied for two species of B. subtilis, and two species of B. cereus against 35 species of Staphylococci which isolated from soil and deferent pathogen samples: ear, urine, Bronchitis excretions, Liver’s wash, CSF, Blood, Abscess, Furuncle, Pus, CV shant, Wounds. The results showed that B. subtilis which isolated from the soil of zone of Al-tall has more antibacterial activity which gives bigger inhibition zone against all strains of Staphylococci which were isolated from soil and pathogens (100%), whereas another strain which was isolated from the soil of zone of ALRabwah didn’t give antibacterial activity against some of these strains of Staphylococci (38.86%, 27.27%). In addition, two species of B. cereus didn’t have any antibacterial activity towards most strains of Staphylococci, except six of it (31.42%). With respect to strains of Staphylococci which was isolated from soil, B. subtilis of Al-tall has more antibacterial activity which give bigger inhibition zone (10-21 mm) against all of strains of Staphylococci which isolated from different soil except S. epidermidis (0 mm) which isolated from Adawee, while another species which isolated from the soil of AL-Rabwah gave antibacterial activity against some of these strains of Staphylococci. In addition, two species of B. cereus had antibacterial activity towards three strains of Staphylococci, but no activity towards four strains of it, and there is a significant difference between B. subtilis1 and Bs2, Bc1, Bc2 in almost strains of Staphylococci (P< 0.05).

2016-01-01

Detection of mecA Gene, vanA Gene in Staphylococcus Aureus and Staphylococcus Epidermidis from Clinical Samples

Journal of Laboratory Diagnostics-Syrian Clinical Laboratory Association : (Issue : 1, 2) (Volume : 8)
2016

Current study aimed to identify S. aureus and S. epidermidis strains, and investigate of methicillin resistant gene mecA and vancomycin vanA gene in these bacteria and detection the antimicrobial resistance. The samples were obtained from three hospitals: Al-Mouwasat, Children, and Dermal Venereal Diseases between August 2013 through June 2014. Forty six strains of S. aureus and S. epidermidis were detected, PCR product appeared a single DNA band for nuc, mecA and vanA genes, which coded areas equal to nearly 270 bp, 533 bp, and 1100 bp respectively. The percentage of presence of mecA gene in S. aureus strains (MRSA) was 15% and vanA gene (VRSA) was 2.5%. The percentages of antibiotic resistance were: AMC (65%), AM (70%), AX (55%), GN (30%), CRO (62.5%), PE (7.5%), OFX (7.5%) and VA (2.5%). The MIC of S. aureus was ranging between 16 – 32 μg ml-1, except resistant strains was >256 μg ml-1 while MIC for vancomycin was ranging between 4 – 8 μg ml-1, except resistant strains was >256 μg ml-1. Whereas the MIC for methicillin of all strains of S. epidermidis was ranging between 8 – 16 μg ml-1.

2015-01-01

Antibacterial properties of crude lyophilized extracts of Bacillus subtilis against Coagulase negative Staphylococci CNS

ASIAN JOURNAL OF MICROBIOLOGY, BIOTECHNOLOGY AND ENVIRONMENTAL SCIENCE : (Issue : 4) (Volume : 17)
2015

The aim of this research was to investigate antimicrobials from crude lyophilized extract and used against CNS strains were isolated from clinical and environmental samples. Six strains were isolated from soil and wastewater. The strains were identified by biochemical and 16S DNA methods, another hand, CNS strains were isolated from patients, healthy skin, soil, and wastewater and identified by biochemical methods. The crude antibacterial production was extracted as 3 d. incubation shaker at 37 oC/150 rev min-1, then centrifugation 12000 rev min-1 for 15 min. at 4oC, and filtrate throw 0.45 μm, next freeze and lyophilized. The crude extracts were run on Diamonsil C18 column (5 μm, 250 × 4.6 mm) in HPLC system to separate the antibiotics, also run on FTIR spectrum. Some crude lyophilized extracts which extracted from B. subtilis gave high activity against some strains of CNS and others are not. Two kinds of antibiotics (Bacitracin A and Polymyxin B) were isolated from B. subtilis and identified to the peptide family. Bacitracin A could function as antibacterial against a wide spectrum of CNS pathogens.

2012-01-01

Bacteria associated with Ear Infections in the National Hospital in Qamishly Syria and their Antimicrobial Resistance

Damascus University Journal for Basic Sciences : (Issue : 1) (Volume : 28)

54 isolates were isolated of the bacteria associated with ear infections which have antimicrobial resistance from the patients in the National Hospital in Qamishly City during the period from 01/08/2008 to 31/10/ 2009. The number of Pseudomonas aeruginosa was high, followed by Staphylococcus aureus. It has been found that the rate of ear infections was lower in male (44.4%) than female (55.5%), and was (48.10%) in the first age category (1- 15 year) more than the second age category (15- 30 year) (31.37%), while the rate of ear infections was (23.5%) in the third age category(30- 60 year ). We found that all the strains of isolated bacteria showed high susceptibility to Imipenem (100%), and all bacteria of Pseudomonas, Klebsiella, Proteus, Enterobacter, showed high sensitivity to ciprofloxacin, levofloxacin (85%), but only staphylococcus (58%). In addition, most isolated bacteria showed intermediate sensitivity to tobramycin, amikacin, gentamycin, and cephalosporinses like ceftazidime, cefaclor and cefotaxime. At the same time, most isolated bacteria showed resistance to Amoxicillin, Ampicillin, Penicillin, Oxacylline, Sulphamethoxasole, erythromycin, vancomycin and tetracyclines.

2012-01-01

Sensitivity and Resistance main Staphylococcus Sp. Isolated of urine Cause UTIs in the National Hospital in Qamishly

Tishreen University Journal -Biological Sciences Series : (Issue : 6) (Volume : 34)
2012

Antimicrobial resistance is a big problem that needs a healthy and serious care. The current study researches 39 staphylococcus isolated from urine of patients and outpatients and the antimicrobial resistance. The research carried out at the National Hospital in Qamishly during the period 2008 - 2009. Results showed that the rate of urinary tract infections was more in females (94.87%) than in males (5.12%) and more in adults (87.17%) than children (12.82%). We found that all the strains of isolated bacteria showed high susceptibility against Imipenem (94.82%), Cefazoline (83.84%), Ceftriaxone (69.23%), Cefaclor (69.2%), Amikacin (64.1%), and Gentamycin (61.5%). In addition, most isolated bacteria showed intermediate Sensitivity of Ciprofloxacin (53.8%), Levofloxacin (51.2%), and rate of sensitivity against Tobramycin (38.4%), Amoxicillin clavulanic acid (25.6%), Pefloxacin (25.6%), Vancomycine (25.6%). At the same time, most of the isolated bacteria showed resistance of Ceftazidime, Cefotaxime, Cefuroxime sodium, Ofloxacin, Cefoxitin, Sulphamethoxasole trimethoprim, Tetracyclines, Erythromycin, Penicillin, Ampicillin, Oxacillin, Amoxicillin

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